|
| 产地 | American |
| 品牌 | AAT Bioquest |
| 货号 | CS1279 |
| 用途 | Research Grade |
| 包装规格 | 2x50 ug Labelings |
| 纯度 | 98%% |
| CAS编号 | |
| 是否进口 | 是 |
Readilink™蛋白标记技术是制备用于荧光成像和流式细胞仪应用的荧光抗体缀合物的最强大，最方便的工具之一。ReadiLink™AF750抗体快速标记试剂盒为制造AlexaFluor®750标记的抗体缀合物提供了最便捷的工具（AlexaFluor®是ThermoFisher Scientific的商标）。此ReadiLink™试剂盒中使用的AF750染料相当稳定，并且对蛋白质氨基具有良好的反应性和选择性。该试剂盒具有用于标记?2x50 ug抗体的所有基本组分。试剂盒中提供的两个AF750染料小瓶中的每一个均经过优化，可标记约50 μg抗体。制备的AF750缀合物（与试剂盒一起使用）无需进一步纯化即可直接用于荧光成像和流式细胞术。它提供了制备AF750标记抗体的最便捷方法。
样品分析方案
工作溶液配制
操作步骤
图1.HeLa细胞的免疫荧光分析，先用ReadiLink™快速AF750抗体标记试剂盒（＃CS1279）制备，然后用或不用小鼠抗微管蛋白染色，然后用Alpha Fluor™750山羊抗小鼠IgG缀合物染色。
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