样品分析方案 

工作溶液配制

操作步骤

图1.分别用和不用小鼠抗微管蛋白染色的HeLa细胞，然后用ReadiLink™Rapid AF488抗体标记试剂盒（Cat＃CS1275）制备的Alpha Fluor™488山羊抗小鼠IgG结合物的免疫荧光分析。

参考文献

A Fluorescent Probe to Unravel Functional Features of Cannabinoid Receptor CB1 in Human Blood and Tonsil Immune System Cells.
Authors: Martín-Fontecha, Mar and Angelina, Alba and Rückert, Beate and Rueda-Zubiaurre, Ainoa and Martín-Cruz, Leticia and van de Veen, Willem and Akdis, Mübeccel and Ortega-Gutiérrez, Silvia and López-Rodríguez, María Luz and Akdis, Cezmi A and Palomares, Oscar
Journal: Bioconjugate chemistry (2018): 382-389

N-terminal and central segments of the type 1 ryanodine receptor mediate its interaction with FK506-binding proteins.
Authors: Girgenrath, Tanya and Mahalingam, Mohana and Svensson, Bengt and Nitu, Florentin R and Cornea, Razvan L and Fessenden, James D
Journal: The Journal of biological chemistry (2013): 16073-84

SNAP-tag based agents for preclinical in vitro imaging in malignant diseases.
Authors: Amoury, Manal and Blume, Tobias and Brehm, Hannes and Niesen, Judith and Tenhaef, Niklas and Barth, Stefan and Gattenl?hner, Stefan and Helfrich, Wijnand and Fitting, Jenny and Nachreiner, Thomas and Pardo, Alessa
Journal: Current pharmaceutical design (2013): 5429-36

Transient focal membrane deformation induced by arginine-rich peptides leads to their direct penetration into cells.
Authors: Hirose, Hisaaki and Takeuchi, Toshihide and Osakada, Hiroko and Pujals, Sílvia and Katayama, Sayaka and Nakase, Ikuhiko and Kobayashi, Shouhei and Haraguchi, Tokuko and Futaki, Shiroh
Journal: Molecular therapy : the journal of the American Society of Gene Therapy (2012): 984-93

Chemical visualization of an attractant peptide, LURE.
Authors: Goto, Hiroaki and Okuda, Satohiro and Mizukami, Akane and Mori, Hitoshi and Sasaki, Narie and Kurihara, Daisuke and Higashiyama, Tetsuya
Journal: Plant & cell physiology (2011): 49-58

Multifunctional surface modification of gold-stabilized nanoparticles by bioorthogonal reactions.
Authors: Li, Xiuru and Guo, Jun and Asong, Jinkeng and Wolfert, Margreet A and Boons, Geert-Jan
Journal: Journal of the American Chemical Society (2011): 11147-53

Simultaneous detection of changes in protein expression and oxidative modification as a function of age and APOE genotype.
Authors: DeKroon, Robert M and Osorio, Cristina and Robinette, Jennifer B and Mocanu, Mihaela and Winnik, Witold M and Alzate, Oscar
Journal: Journal of proteome research (2011): 1632-44

Site-specific labeling of proteins for single-molecule FRET measurements using genetically encoded ketone functionalities.
Authors: Lemke, Edward A
Journal: Methods in molecular biology (Clifton, N.J.) (2011): 3-15

Transmission electron microscopy characterization of fluorescently labelled amyloid β 1-40 and α-synuclein aggregates.
Authors: Anderson, Valerie L and Webb, Watt W
Journal: BMC biotechnology (2011): 125

Distribution of nerve endings in the human dorsal radiocarpal ligament.
Authors: Tomita, Kazunari and Berger, Evelyn J and Berger, Richard A and Kraisarin, Jirachart and An, Kai-Nan
Journal: The Journal of hand surgery (2007): 466-73