![]() |
产地 | American |
品牌 | AAT Bioquest |
货号 | CS1278 |
用途 | Research Grade |
包装规格 | 2x50 ug Labelings |
纯度 | 98%% |
CAS编号 | |
是否进口 | 是 |
Readilink™蛋白标记技术是制备用于荧光成像和流式细胞仪应用的荧光抗体缀合物的最强大,最方便的工具之一。ReadiLink™AF647抗体快速标记试剂盒为制造AlexaFluor®647标记的抗体缀合物提供了最便捷的工具(AlexaFluor®是ThermoFisher Scientific的商标)。此ReadiLink™试剂盒中使用的AF647染料相当稳定,并且对蛋白质氨基具有良好的反应性和选择性。该试剂盒具有用于标记?2x50 ug抗体的所有基本组分。试剂盒中提供的两个AF647染料小瓶中的每一个均经过优化,可标记约50 μg抗体。制备的AF647缀合物(与试剂盒一起使用)无需进一步纯化即可直接用于荧光成像和流式细胞术。它提供了制备AF647标记抗体的最便捷方法。
样品分析方案
工作溶液配制
操作步骤
图1.分别用和不用小鼠抗微管蛋白染色的HeLa细胞,然后用ReadiLink™Rapid AF647抗体标记试剂盒(#CS1278)制备的Alpha Fluor™647山羊抗小鼠IgG结合物的免疫荧光分析。
参考文献
Deciphering Design Principles of F?rster Resonance Energy Transfer-Based Protease Substrates: Thermolysin-Like Protease from Geobacillus stearothermophilus as a Test Case.
Authors: Ripp, Sophie and Turunen, Petri and Minot, Ethan D and Rowan, Alan E and Blank, Kerstin G
Journal: ACS omega (2018): 4148-4156
An enzymatically-sensitized sequential and concentric energy transfer relay self-assembled around semiconductor quantum dots.
Authors: Samanta, Anirban and Walper, Scott A and Susumu, Kimihiro and Dwyer, Chris L and Medintz, Igor L
Journal: Nanoscale (2015): 7603-14
Collective localized surface plasmons for high performance fluorescence biosensing.
Authors: Bauch, Martin and Dostalek, Jakub
Journal: Optics express (2013): 20470-83
Facile synthesis of symmetric, monofunctional cyanine dyes for imaging applications.
Authors: Ying, Lai-Qiang and Branchaud, Bruce P
Journal: Bioconjugate chemistry (2011): 865-9
Effects of ozone exposure during microarray posthybridization washes and scanning.
Authors: Byerly, Steve and Sundin, Kyle and Raja, Rajiv and Stanchfield, Jim and Bejjani, Bassem A and Shaffer, Lisa G
Journal: The Journal of molecular diagnostics : JMD (2009): 590-7
Comparison of Alexa Fluor and CyDye for practical DNA microarray use.
Authors: Ballard, Joanne L and Peeva, Violet K and deSilva, Christopher J S and Lynch, Jessica L and Swanson, Nigel R
Journal: Molecular biotechnology (2007): 175-83
Possible sources of dye-related signal correlation bias in two-color DNA microarray assays.
Authors: Cox, W Gregory and Beaudet, Matthew P and Agnew, Jakyoung Y and Ruth, Jerry L
Journal: Analytical biochemistry (2004): 243-54
Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates.
Authors: Berlier, Judith E and Rothe, Anca and Buller, Gayle and Bradford, Jolene and Gray, Diane R and Filanoski, Brian J and Telford, William G and Yue, Stephen and Liu, Jixiang and Cheung, Ching-Ying and Chang, Wesley and Hirsch, James D and Beechem, Joseph M and Haugland, Rosaria P and Haugland, Richard P
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2003): 1699-712
Surface plasmon field-enhanced fluorescence spectroscopy studies of the interaction between an antibody and its surface-coupled antigen.
Authors: Yu, Fang and Yao, Danfeng and Knoll, Wolfgang
Journal: Analytical chemistry (2003): 2610-7
Improved fluoroimmunoassays using the dye Alexa Fluor 647 with the RAPTOR, a fiber optic biosensor.
Authors: Anderson, George P and Nerurkar, Nandan L
Journal: Journal of immunological methods (2002): 17-24