物品单位 | 价格 | 品牌 |
---|---|---|
支 | 1836 | AAT Bioquest |
产地 | American |
品牌 | AAT Bioquest |
货号 | CS1277 |
用途范围 | Research Grade |
纯度 | 98%% |
CAS编号 | |
规格 | 2x50 ug Labelings |
是否进口 | 是 |
Readilink™蛋白标记技术是制备用于荧光成像和流式细胞仪应用的荧光抗体缀合物的最强大,最方便的工具之一。ReadiLink™AF594抗体快速标记试剂盒为制造AlexaFluor®594标记的抗体缀合物提供了最便捷的工具(AlexaFluor®是ThermoFisher Scientific的商标)。此ReadiLink™试剂盒中使用的AF594染料相当稳定,并且对蛋白质氨基具有良好的反应性和选择性。该试剂盒具有用于标记?2x50 ug抗体的所有基本组分。试剂盒中提供的两个AF594染料小瓶中的每一个均经过优化,可标记约50 μg抗体。制备的AF594缀合物(与试剂盒一起使用)无需进一步纯化即可直接用于荧光成像和流式细胞术。它提供了制备AF594标记抗体的最便捷方法。
样品分析方案
工作溶液配制
操作步骤
图1.分别用和不用小鼠抗微管蛋白染色的HeLa细胞,然后用ReadiLink™Rapid AF594抗体标记试剂盒(#CS1277)制备的Alpha Fluor™594山羊抗小鼠IgG结合物的免疫荧光分析。
参考文献
Fluorescently-labeled fremanezumab is distributed to sensory and autonomic ganglia and the dura but not to the brain of rats with uncompromised blood brain barrier.
Authors: Noseda, Rodrigo and Schain, Aaron J and Melo-Carrillo, Agustin and Tien, Jason and Stratton, Jennifer and Mai, Fanny and Strassman, Andrew M and Burstein, Rami
Journal: Cephalalgia : an international journal of headache (2020): 229-240
Super blinking and biocompatible nanoprobes based on dye doped BSA nanoparticles for super resolution imaging.
Authors: Zong, Shenfei and Pan, Fengmei and Zhang, Ruohu and Chen, Chen and Wang, Zhuyuan and Cui, Yiping
Journal: Nanotechnology (2019): 065701
[Sepsis impairs aggregation of nicotinic acetylcholine receptors on murine skeletal muscle cell membranes by inhibiting AKT/GSK3β phosphorylation].
Authors: Li, Tianmei and Liu, Li and Wang, Xiaobin
Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (2019): 1337-1343
Astrocytic Atrophy Following Status Epilepticus Parallels Reduced Ca2+ Activity and Impaired Synaptic Plasticity in the Rat Hippocampus.
Authors: Plata, Alex and Lebedeva, Albina and Denisov, Pavel and Nosova, Olga and Postnikova, Tatiana Y and Pimashkin, Alexey and Brazhe, Alexey and Zaitsev, Aleksey V and Rusakov, Dmitri A and Semyanov, Alexey
Journal: Frontiers in molecular neuroscience (2018): 215
Bioorthogonal click chemistry for fluorescence imaging of choline phospholipids in plants.
Authors: Paper, Janet M and Mukherjee, Thiya and Schrick, Kathrin
Journal: Plant methods (2018): 31
Inhibitory Projections in the Mouse Auditory Tectothalamic System.
Authors: Clarke, Blaise A and Lee, Charles C
Journal: Brain sciences (2018)
Super-resolution Microscopical Localization of Dopamine Receptors 1 and 2 in Rat Hippocampal Synaptosomes.
Authors: Miklosi, Andras G and Del Favero, Giorgia and Bulat, Tanja and H?ger, Harald and Shigemoto, Ryuichi and Marko, Doris and Lubec, Gert
Journal: Molecular neurobiology (2018): 4857-4869
[Adenoid cystic carcinoma cells produce exosomes that promote tumor cell proliferation].
Authors: Liu, Xiaohao and Wang, Fangyuan and Hou, Jin and Zhang, Leitao and Chen, Zhifeng and Yin, Xuemin
Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (2018): 1008-1013
Collateralization of projections from the paraventricular nucleus of the thalamus to the nucleus accumbens, bed nucleus of the stria terminalis, and central nucleus of the amygdala.
Authors: Dong, Xinwen and Li, Sa and Kirouac, Gilbert J
Journal: Brain structure & function (2017): 3927-3943
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