15926423062
|
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| 产地 | American |
| 品牌 | AAT Bioquest |
| 货号 | CS13555 |
| 用途范围 | Research Grade |
| 纯度 | 98%% |
| 规格 | 1 mg |
| 是否进口 | 是 |
ACE2（血'管紧张素转换酶2）是一种金属蛋白酶，需要将二价阳离子置于活性位点才能进行催化。它具有围绕其三价功能的多种生理作用：肾素-血'管紧张素系统的负调节剂，氨基酸转运的促进剂以及严重的急性呼'吸系'统综合症'冠'状'病'毒（SARS-CoV）和S'A'RS-C'oV-2受体。ACE2最近被确定为S'ARS-CoV-2受体，是冠’状'病'毒疾病的传染源，在免疫，炎'症，ACE2和心'血'管疾'病之间提供了关键的联系。尽管与SARS-CoV具有密切的进化关系，但SA'RS-CoV-2的受体结合域在几个关键氨基酸残基上有所不同，从而与人ACE2受体具有更强的结合亲和力，这可能是SA'RS-CoV-2致病性更大的原因。由S'ARS-CoV-2结合后，ACE2功能的丧失是由内吞作用以及蛋白水解切割和加工的激活驱动的。ACE2系统是防止心'力'衰'竭（包括心'肌'梗'塞和高'血'压）以及肺部疾病和糖'尿'病的重要保护途径。ACE2控制肠营'养不'良和血'管通透性已成为肺'动'脉'高'压和糖'尿'病性心血'管并'发'症的重要机制。Mca-APK（Dnp）ACE2底物是一种FRET肽，用于测量细胞和组织中的酶活性。它使用DNP作为淬灭剂分子来淬灭甲氧基香豆素（Mca）的荧光。当酶切割脯氨酸-赖氨酸残基并恢复Mca的荧光时，这种相互作用被消除。这种荧光底物比常用的基于HPLC分离的方法具有更大的灵活性和更高的通量。
样品分析方案
简要概述
准备测试样品和空白样品（50 μL）
根据需要用抑制剂处理样品
加入Mca-APK（Dnp）ACE2底物工作溶液（50 μL）
在37°C下孵育样品
在Ex / Em = 320/430 nm（截止= 420 nm）下检测荧光
储备溶液配制
操作步骤
参考文献
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15926423062
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