产品描述

Ansamitocin p-3 (Maytansinol isobutyrate, NSC292222) is a potent tubulin polymerization inhibitor with IC50 of 3.4 μM.

靶点活性

Others

细胞实验

Cell lines: A31 and KB. Concentrations: Dissolved in DMSO, final concentrations ~10 μM. Incubation Time: ~24 hours. Method: After synchronized, cells are exposed to various Ansamitocin p-3 concentrations for ~24 hours. Cells are labeled with [3H]thymidine (5 Ci/mM, 1 μCi/mL) in 1 mL medium. After pulse-labeling at 37 °C for 1 hour, the cells on coverslips are fixed with methanol:acetic acid (3:1). The acidsoluble fraction is washed out from the cells, and a liquid scintillation counter is used to determine the radio activity of each coverslip.

激酶实验

Polymerization inhibition assay: After the addition of 1M Tris buffer (pH 8.4, for blank) or 100 μL of various concentrations of Ansamitocin p-3 solution (GTP minus MES buffer), to 400 μL of bovine tubulin solution (1 mg/mL in cold MES buffer), maintained at 0 °C for 10 to 15 minutes, the mixture is warmed in a water bath at 37 °C for 30 to 60 minutes. Tubulin polymerization is followed by an increase in turbidity of the mixture during warming. The turbidity measurement is performed at 460 nm with a Hitachi type 101 spectrophotometer.

动物实验

Animal Models: C57BL/6 mice bearing C1498, or B16 cells, Female DBA/2 mice bearing L1210, P815, or P388 cells, BALB/c mice bearing MOPC-104E cells, and ICR mice bearing sarcoma 180 and EAC. Dosages: about 200 μg/kg. Administration: Administered i.v. or i.p. daily.

别名

Antibiotic C 15003P3 , NSC292222 , Maytansinol isobutyrate , 安丝菌素 P 3 , 安丝菌素 P 3 , NSC292222 , Maytansinol isobutyrate , Antibiotic C 15003P3

分子量

635.14

分子式

C32H43ClN2O9

CAS No.

66584-72-3

存储

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

溶解度

DMSO: 100 mg/ml (157.45 mM)

Ethanol: 55 mg/ml (86.6 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

参考文献

1. Ootsu K, et al. Cancer Res, 1980, 40(5), 1707-1717.

2. Suwanborirux K, et al. Experientia, 1990, 46(1), 117-120.

3. Young DH, et al. J Biomol Screen, 2006, 11(1), 82-89.

4. Edwards A, et al. Dis Model Mech, 2011, 4(4), 496-503.

5. Jubina B Venghateri, et al. PLoS One. 2013 Oct 4;8(10):e75182.

Note

For research use only .