产品描述

5-Ethynyl-2'-deoxyuridine (EdU) is a nucleoside analog of thymidine that is used to monitor de novo DNA synthesis through click chemistry.It is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs.

靶点活性

Alkyl-Chain

体外活性

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].Like 5-bromo-2’-deoxyuridine (BrdU), EdU is incorporated into DNA during active DNA synthesis. It can then be detected using a fluorescent azide probe and copper catalysis using 1,3-dipolar cycloaddition.Modified nucleosides, including EdU, can be cytotoxic when added to cells for prolonged periods[2].5-Ethynyl-2'-deoxyuridine (EdU) staining is a fast, sensitive and reproducible method to study cell proliferation in the central nervous system[3]

别名

5-乙炔基-2‘-脱氧尿苷

纯度

98%

分子量

252.22

分子式

C11H12N2O5

CAS No.

61135-33-9

存储

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

溶解度

H2O: 25 mg/mL (95.15 mM), Need ultrasonic

DMSO: 120 mg/mL (475.77 mM), Need ultrasonic

( < 1 mg/ml refers to the product slightly soluble or insoluble )

参考文献

1. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562

2. Cavanagh B L , Walker T , Norazit A , et al. Thymidine Analogues for Tracking DNA Synthesis[J]. Molecules, 2011, 16(9):7980-7993.

3. Zeng C , Pan F , Jones L A , et al. Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the a’dult nervous system.[J]. Brain Research, 2010, 1319(none):21-32.

Note

For research use only.