|别名||1-Methylpseudouridine , N1-甲基-假尿苷|
N1-Methylpseudouridine is a methylpseudouridine and enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density in mRNA.
In HEK293T cells, Incorporation of N1-Methylpseudouridine into mRNA modifies mRNAs produced higher amounts of luc than the standard Luc mRNA . Incorporation of N1-Methylpseudouridine nucleoside modification in both Luc and GFP mRNA enhances the initiation step of translation, in part by suppressing eIF2α phosphorylation. In addition, polysome formation and growth on the N1-Methylpseudouridine-containing Luc mRNA is enhanced due to the reduction of elongation rate.
mRNAs containing the N1-Methylpseudouridine modification alone and/or in combination with 5-methylcytidine (m5C) outperformed the current state-of-the-art pseudouridine (Ψ) and/or m5C/Ψ-modified mRNA platform by providing up to ~44-fold (when comparing double modified mRNAs) or ~13-fold (when comparing single modified mRNAs) higher reporter gene expression upon transfection into cell lines or mice, respectively. We show that (m5C/)N1-Methylpseudouridine-modified mRNA resulted in reduced intracellular innate immunogenicity and improved cellular viability compared to (m5C/)Ψ-modified mRNA upon in vitro transfection.
1-Methylpseudouridine , N1-甲基-假尿苷
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
H2O: 40 mg/mL (154.90 mM)
DMSO: 100 mg/mL (387.25 mM)
( < 1 mg/ml refers to the product slightly soluble or insoluble )
1. Svitkin YV, et al. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density. Nucleic Acids Res. 2017 Jun 2;45(10):6023-6036.
2. Andries O, et al. N(1)-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice. J Control Release. 2015 Nov 10;217:337-44.
For research use only.