产品描述 

Foscarnet sodium is an antiviral agent used in the treatment of cytomegalovirus retinitis. Foscarnet also shows activity against human herpesviruses and HIV. 

靶点活性 

Reverse Transcriptase 

体外活性 

Foscarnet Sodium is able to penetrate into cells and inhibit viral replication by inhibiting viral DNA synthesis. Foscarnet Sodium (300 μM) displays a more than 95% inhibition on HTLV-III reverse transcriptase activity in H9 cells infected with HTLV-III. [1] Foscarnet Sodium has a low cellular toxicity, but shows an inhibition of cell proliferation at high concentrations, which is reversed after drug removal. Incubation 0.5-1 mM Foscarnet Sodium with HeLa cells or primary human lung fibroblast for 24-48 hr inhibited 50% DNA synthesis and/or cell proliferation. [1] Foscarnet Sodium is able to bind with the heparin-binding domain of FGF2 and inhibits FGF2 induced cell proliferation. Foscarnet Sodium (25 μM) treating HUVEC cells for 48 hr reduced FGF2-induced cellular proliferation by 20%. Foscarnet Sodium (0.5-1 mM) reduced the 24h proliferation rate of CAL-62 cell, a Human thyroid anaplastic carcinoma cell line which expresses FGF2, to nearly 80%. [2] 

体内活性 

Foscarnet Sodium display an efficient therapy effect on animal with virus diseases, such as cutaneous infection, herpes keratitis, genital infection, or systemic and interacerebral infection. For example, Foscarnet Sodium (100 mM) applied at initiated 48 h post inoculation with twice daily applications of 30 μL for 4 days, displays a more than 70% reduction in cumulative score after inoculation with HSV-1 strain C42. Foscarnet Sodium (20 mg/kg) given as an aerosol to mice infected with murine CMV caused a reduction of 60% in lung titers. [1] 

激酶实验 

Reverse transcriptase assays: Reverse transcriptase assays are carried out in a reaction mixture (50 μL) containing 50 mM Tris-HCl (pH 7.5), 5 mM DTT, 100 mM potassium chloride, 0.01% Triton X-100 or NP40, 10 μg/ml (dT)15.(A)n as template primer and [3H] deoxythymidine triphospahate. The reaction mixture is incubated for 1 hr at 37 ℃ and stopped by the addition of 50 μg of yeast tRNA and 2 mL of 10% solution of trichloroacetic acid containing 1 mM sodium pyrophosphate. The samples are filtered on filters (0.45 μm), washed first with 5% TCA solution for 5 times and then with 2 ml of 70% ethanol. The filters are dried, scintillation fluid is added and the radioactivity counted in a counter. 
细胞实验 

CAL-62 cells are placed on 96 well plates at 8×103 cells/well in 100 μL DMEM for 24 h. Then medium is replaced by fresh DMEM with or without foscarnet. After 48 h of incubation, 20 μL of CellTiter 96AQ solution is added to the cells and incubated for 2-4 h. The quantity of formazan product as measured by the absorbance at 492 nm.(Only for Reference) Cell lines: Human thyroid anaplastic carcinoma cell line, CAL-62 cell 

别名 膦甲酸钠 , Phosphonoformate 

纯度 

≥95%

191.95 
分子式 

CNa₃O₅P 
CAS No. 

63585-09-1 

存储
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
溶解度
DMSO: 7 mg/mL (36.47 mM)
Ethanol: <1 mg/mL
Water: 3 mg/mL (15.63 mM)
( < 1 mg/ml refers to the product slightly soluble or insoluble )
参考文献
1. Oberg B. Pharmacol Ther, 1989, 40(2), 213-285.2. Rose K. Biomed Pharmacother, 2013, 67(1), 53-57.3. Sarin PS, et al. Biochem Pharmacol, 1985, 34(22), 4075-4079.4. Alenius S, et al. Antimicrob Agents Chemother, 1978, 14(3), 408-413.

For research use only.