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V5 Epitope Tag Peptide
物品单位 价格 品牌
861 Chemstan
  • 产地:China
  • 型号:5mg
  • 货号:CSGP10153
  • 发布日期: 2022-10-10
  • 更新日期: 2022-10-11
产品详细说明
产地 China
品牌 Chemstan
货号 CSGP10153
用途范围 Research Grade
纯度 >98%%
CAS编号
规格 5mg
是否进口
产品描述
The recognized V5 epitope represents 95GKPIPNPLLGLDST108 of RNA polymerase α subunit of simian parain?uenza virus type 5. This short peptide sequence was chosen because high-af?nity antibodies can be reliably produced in many different species1.
A 14-amino-acid V5 epitope derived from simian parain?uenza virus type 5 (Southernet al., 1991) was inserted into the C terminus of the capsid protein (before the stop codon ofORF2) to construct a recombinant marker virus. We demonstrated that the V5 epitope was displayed on the surface ofthe capsid protein. Furthermore, the recombinant marker virus behaved similarly to the parental virus in vitro and in mice, and could be differentiated from the parental virus via polymerase chain reaction (PCR) and serological methods2, 3.
A V5/His-epitope tag was added to the amino terminus of each coding sequence to distinguish the plasmid-encoded protein from endogenous LRRK2. Plasmids were transiently transfected into COS7 cells, and cell lysates were analyzed by SDS-PAGE and Western blotting with antibodies speci?c for the V5 epitope tag or LRRK24.
References:
1. Zhou,W.H.,Dong, L.L., Ginsburg,D.,Bouhassira, E.E., Tsai,H.M.,2005. Enzymatically active ADAMTS 13 variants are not inhibited by anti-ADAMTS 13 autoantibodies: a novel therapeutic strategy? J. Biol. Chem. 280, 39934–39941.
2. Southern,J.A.,Young,D.F.,Heaney, F.,Baumgartner,W.K.,Randall,R.E.,1991.Identi?cation of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J. Gen. Virol. 72,1551–1557
3. L. Huang, Y. Lu et al. Construction and biological characterisation of recombinant porcine circovirus type 2 expressing the V5 epitope tag. Virus Research. 161 (2011) 115–123
4. D. MacLeod,J. Dowman, The Familial Parkinsonism Gene LRRK2Regulates Neurite Process Morphology. Neuron 52, 587–593, 2006
Chemical Properties

Cas No. 

N/A 

SDF  

别名 

H-Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-OH 

化学名 

V5 Epitope Tag 

Canonical SMILES CCC(C)C(C(=O)N1CCCC1C(=O)NC(CC(=O)N)C(=O)N2CCCC2C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NCC(=O)NC(CC(C)C)C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)NC(C(C)O)C(=O)O)NC(=O)C3CCCN3C(=O)C(CCCCN)NC(=O)CN 

分子式 

C64H108N16O20 

分子量 

1421.64 

溶解度 

≥ 71.082mg/mL in DMSO, ≥ 107.2 mg/mL in EtOH, ≥ 55.4 mg/mL in H2O 

储存条件 

Desiccate at -20°C 

General tips 

For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. 

Shipping Condition 

Evaluation sample solution : ship with blue ice

All other available size: ship with RT , or blue ice upon request 

Purity: 

>98.00%

Note
For research use only .

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