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Anti RAS(A146P) Mouse Monoclonal Antibody
  • 品牌:Chemstan
  • 产地:China
  • 型号:100ug
  • 货号:CST26478
  • 价格: ¥4800/支
  • 发布日期: 2023-06-08
  • 更新日期: 2024-07-18
产品详请
产地 China
品牌 Chemstan
货号 CST26478
用途 Research Grade
纯度 >95% by SDS-PAGE.%
规格 100ug
是否进口
Catalog Number:  CST26478
Gene Symbol:  H-Ras; K-Ras; N-Ras
Description:   Anti RAS(A146P) Mouse Monoclonal Antibody
Background:   The A146P mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146P mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
Immunogen:   A synthetic peptide from the internal region of Ras which includes the mutation of A146P, human origin.
Applications:   ELISA, IF, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
IF: 1:50-1:100 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes A146P mutant, but not wild-type RAS of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Immunofluorescence:  
Immunofluorescence of cells expressing KRas proteins with anti-KRAS(A146P) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRAS(A146P) plasmid , pCDNA3-GFP KRas(WT) plasmid, pCDNA3-GFP KRas(A146V) plasmid or pCDNA3-GFP KRas(A146T) plasmid, then fixed and stained with anti-KRAS(A146P) monoclonal antibody (Cat. #26478).

Note:
For research use only .

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