15926423062
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| 产地 | China |
| 品牌 | Chemstan |
| 货号 | CST26478 |
| 用途范围 | Research Grade |
| 纯度 | >95% by SDS-PAGE.% |
| 规格 | 100ug |
| 是否进口 | 否 |
| Catalog Number: CST26478 |
| Gene Symbol: H-Ras; K-Ras; N-Ras |
| Description: Anti RAS(A146P) Mouse Monoclonal Antibody |
| Background: The A146P mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146P mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. |
| Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of A146P, human origin. |
| Applications: ELISA, IF, IHC |
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Recommended Dilutions: ELISA: 1:1000-1:5000 IF: 1:50-1:100 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes A146P mutant, but not wild-type RAS of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Immunofluorescence: |
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Immunofluorescence of cells expressing KRas proteins with anti-KRAS(A146P) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRAS(A146P) plasmid , pCDNA3-GFP KRas(WT) plasmid, pCDNA3-GFP KRas(A146V) plasmid or pCDNA3-GFP KRas(A146T) plasmid, then fixed and stained with anti-KRAS(A146P) monoclonal antibody (Cat. #26478).
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15926423062
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