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Anti RAS(G13V) Mouse Monoclonal Antibody
  • 品牌:Chemstan
  • 产地:China
  • 型号:100ug
  • 货号:CST26477
  • 价格: ¥4800/支
  • 发布日期: 2023-06-08
  • 更新日期: 2024-07-18
产品详请
产地 China
品牌 Chemstan
货号 CST26477
用途 Research Grade
纯度 >95% by SDS-PAGE.%
规格 100ug
是否进口
Catalog Number:  CST26477
Gene Symbol:  H-Ras; K-Ras; N-Ras
Description:   Anti RAS(G13V) Mouse Monoclonal Antibody
Background:   Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G13V mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
Immunogen:   A synthetic peptide from the internal region of Ras which includes the mutation of G13V, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:1000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes G13V mutant, but not wild-type RAS of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant Ras proteins. Purified His-tagged RAS(G13V) protein (lane 1), corresponding wildtype protein (lane 2), His-tagged Ras(G13S) protein (lane 3), His-tagged Ras(G13C) protein (lane 4), His-tagged Ras(G13R) protein (lane 5), His-tagged Ras(G13A) protein (lane 6) were blotted with Anti RAS(G13V) monoclonal antibody (Cat. #26477).
Immunofluorescence:  
Immunofluorescence of cells expressing Ras proteins with Anti RAS(G13V) antibody. HEK293T cells were transfected with pCDNA3-GFP-RAS(G13V) plasmid ,pCDNA3-GFP Ras(WT) plasmid, pCDNA3-GFP Ras(G13S) plasmid, pCDNA3-GFP Ras(G13C) plasmid, pCDNA3-GFP Ras(G13R) plasmid, pCDNA3-GFP Ras(G13A) plasmid or pCDNA3-GFP Ras(G13D) plasmid, then fixed and stained with Anti RAS(G13V) monoclonal antibody (Cat. #26477).

Note:
For research use only .

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