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Anti NOTCH1(M2011R) Mouse Monoclonal Antibody
  • 品牌:Chemstan
  • 产地:China
  • 型号:100ug
  • 货号:CST26444
  • 价格: ¥4800/支
  • 发布日期: 2023-06-08
  • 更新日期: 2024-04-12
产品详请
产地 China
品牌 Chemstan
货号 CST26444
用途范围 Research Grade
纯度 >95% by SDS-PAGE.%
规格 100ug
是否进口
Catalog Number:  CST26444
Gene Symbol:  NOTCH1, AOS5, AOVD1,TAN1, hN1
Description:   Anti NOTCH1(M2011R) Mouse Monoclonal Antibody
Background:   NOTCH1 is a human gene encoding a single-pass transmembrane receptor. It is a member of the NOTCH family. NOTCH family members play a role in a variety of developmental processes by controlling cell fate decisions. Diseases associated with NOTCH1 include aortic valve disease 1 and adams-oliver syndrome 5, and so on. An important paralog of this gene is NOTCH2.
Immunogen:   A synthetic peptide from the internal region of NOTCH1 which includes the mutation of M2011R, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:2000 
WB: 1:500-1:1000 
IF: 1:50-1:100 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes M2011R mutant, but not wild-type NOTCH1 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant NOTCH1(M2011R) and wildtype proteins. Purified His-tagged NOTCH1(M2011R) protein (lane1) and corresponding wild-type protein (lane2) were blotted with Anti NOTCH1(M2011R) monoclonal antibody (Cat. #26444).
Immunofluorescence:  


Note:
For research use only .

Immunofluorescence of cells expressing NOTCH1 proteins with Anti NOTCH1(M2011R) antibody. HEK293T cells were transfected with pCDNA3-GFP NOTCH1(WT) plasmid (left column) or pCDNA3-GFP NOTCH1(M2011R) plasmid (right column), then fixed and stained with Anti NOTCH1(M2011R) monoclonal antibody (Cat. #26444).

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