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Anti NFE2L2(D29G) Mouse Monoclonal Antibody
  • 品牌:Chemstan
  • 产地:China
  • 型号:100ug
  • 货号:CST26331
  • 价格: ¥4800/支
  • 发布日期: 2023-06-08
  • 更新日期: 2024-07-18
产品详请
产地 China
品牌 Chemstan
货号 CST26331
用途 Research Grade
纯度 >95% by SDS-PAGE.%
规格 100ug
是否进口
Catalog Number:  CST26331
Gene Symbol:  NFE2L2, NRF2
Description:   Anti NFE2L2(D29G) Mouse Monoclonal Antibody
Background:   NFE2L2 protein is encoded by NFE2L2 gene. It is a basic leucine zipper protein that regulates the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation. Several drugs that stimulate the NFE2L2 pathway are being studied for treatment of diseases that are caused by oxidative stress.
Immunogen:   A synthetic peptide from the internal region of NFE2L2 which includes the mutation of D29G, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:2000 
WB: 1:500-1:1000 
IHC: 1:50-1:100
Concentration:    1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes D29G mutant, but not wild-type NFE2L2 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant NFE2L2(D29G) and wildtype proteins. Purified His-tagged NFE2L2(D29G) protein (lane2) and corresponding wild-type protein (lane1) were blotted with Anti NFE2L2(D29G) monoclonal antibody (Cat. #26331).
Immunofluorescence:  
Immunofluorescence of cells expressing NFE2L2 proteins with Anti NFE2L2(D29G) antibody. HEK293T cells were transfected with pCDNA3-GFP NFE2L2(WT) plasmid (left column) or pCDNA3-GFP NFE2L2(D29G) plasmid (right column), then fixed and stained with Anti NFE2L2(D29G) monoclonal antibody (Cat. #26331).

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