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Anti Active RhoA Mouse Monoclonal Antibody
  • 品牌:Chemstan
  • 产地:China
  • 型号:100ug
  • 货号:CST26904
  • 价格: ¥4800/支
  • 发布日期: 2023-06-08
  • 更新日期: 2024-05-24
产品详请
产地 China
品牌 Chemstan
货号 CST26904
用途 IP, IHC
纯度 >95% by SDS-PAGE.%
规格 100ug
是否进口
Catalog Number:  CST26904
Gene Symbol:  RHOA
Description:   Anti Active RhoA Mouse Monoclonal Antibody
Background:   Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction.
Immunogen:   Recombinant full length protein of active RhoA
Applications:   IP, IHC
Recommended Dilutions:   1 μg for 1~2 mg total cellular proteins for IP; 
                                           1:100 dilution for IHC
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG2b
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active RhoA antibody recognizes active RhoA from vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Immunoprecipitation/Western Blot:  


Note:
For research use only .

IP/WB analysis of active RhoA protein. Purified recombinant RhoA proteins wer
e loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated
with anti active RhoA mouse monoclonal antibody (Cat. # 26904). After SDS/PAGE, the
membrane filter was probed with anti RhoA mouse monoclonal antibody (Cat. # 26007).


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