|
Catalog Number: CST26422
|
|
Gene Symbol: SMAD4, DPC4, JIP, MADH4, MYHRS
|
|
Description: Anti SMAD4(R361H) Mouse Monoclonal Antibody
|
|
Background: SMAD4 family member 4, also known as SMAD4, is a protein that in human is encoded by the SMAD4 gene. It is often found mutated in many cancers. It acts as a tumor suppressor that functions in the regulation of the TGF-β signal transduction pathway, which negatively regulates growth of epithelial cells and the extracellular matrix (ECM). SMAD4 alterations have been found in multiploid colorectal cancer and pancreatic carcinoma. It is found inactivated in at least 50% of pancreatic cancers. It is also found mutated in the autosomal dominantdisease juvenile polyposis syndrome (JPS).
|
|
Immunogen: A synthetic peptide from the internal region of SMAD4 which includes the mutation of R361H, human origin.
|
|
Applications: ELISA, WB, IHC
|
Recommended Dilutions:
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100
|
|
Concentration: 1 mg/ml
|
|
Host: Mouse
|
|
Format: Liquid
|
|
Clonality: Monoclonal
|
|
Isotype:
|
|
Purity: Purified from ascites
|
|
Storage buffer:
|
|
Preservative: no
|
|
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
|
|
Species Reactivity: Recognizes R361H mutant, but not wild-type SMAD4 of vertebrates.
|
|
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
|
|
|
|
Western blot:
|
