15926423062
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| 产地 | China |
| 品牌 | Chemstan |
| 货号 | CST26422 |
| 用途范围 | Research Grade |
| 纯度 | >95% by SDS-PAGE.% |
| 规格 | 100ug |
| 是否进口 | 否 |
| Catalog Number: CST26422 |
| Gene Symbol: SMAD4, DPC4, JIP, MADH4, MYHRS |
| Description: Anti SMAD4(R361H) Mouse Monoclonal Antibody |
| Background: SMAD4 family member 4, also known as SMAD4, is a protein that in human is encoded by the SMAD4 gene. It is often found mutated in many cancers. It acts as a tumor suppressor that functions in the regulation of the TGF-β signal transduction pathway, which negatively regulates growth of epithelial cells and the extracellular matrix (ECM). SMAD4 alterations have been found in multiploid colorectal cancer and pancreatic carcinoma. It is found inactivated in at least 50% of pancreatic cancers. It is also found mutated in the autosomal dominantdisease juvenile polyposis syndrome (JPS). |
| Immunogen: A synthetic peptide from the internal region of SMAD4 which includes the mutation of R361H, human origin. |
| Applications: ELISA, WB, IHC |
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Recommended Dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes R361H mutant, but not wild-type SMAD4 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
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Western blot analysis of recombinant SMAD4(R361H) and wildtype proteins. Purified His-tagged SMAD4(R361H) protein (lane2) and corresponding wild-type protein (lane1) were blotted with Anti SMAD4(R361H) monoclonal antibody (Cat. #26422). |
| Immunofluorescence: |
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Immunofluorescence of cells expressing SMAD4 proteins with Anti SMAD4(R361H) antibody. HEK293T cells were transfected with pCDNA3-GFP-SMAD4(R361H) plasmid pCDNA3-GFP SMAD4(WT) plasmid, pCDNA3-GFP SMAD4(R361S) plasmid or pCDNA3-GFP SMAD4(R361C) plasmid, then fixed and stained with Anti SMAD4(R361H) monoclonal antibody (Cat. #26422).
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15926423062
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