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Anti SMO(A68V) Mouse Monoclonal Antibody
  • 品牌:Chemstan
  • 产地:China
  • 型号:100ug
  • 货号:CST26425
  • 价格: ¥4800/支
  • 发布日期: 2023-06-08
  • 更新日期: 2024-12-06
产品详请
产地 China
品牌 Chemstan
货号 CST26425
用途 Research Grade
包装规格 100ug
纯度 >95% by SDS-PAGE.%
CAS编号
是否进口
Catalog Number:  CST26425
Gene Symbol:  SMO; MRD21
Description:   Anti SMO(A68V) Mouse Monoclonal Antibody
Background:   Smoothened is a protein that in humans is encoded by the SMO gene. Smoothened is a G protein-coupled receptor that is a component of the hedgehog signaling pathway and is conserved from flies to humans. It is the molecular target of the teratogen cyclopamine. SMO can function as an oncogene. Activating SMO mutations can lead to unregulated activation of the hedgehog pathway and cancer
Immunogen:   A synthetic peptide from the internal region of SMO which includes the mutation of A68V, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:1000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes A68V mutant, but not wild-type SMO of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant SMO(A68V) and wild-type proteins. Purified His-tagged SMO(A68V) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti SMO(A68V) monoclonal antibody (Cat. #26425).
Immunofluorescence:  
Immunofluorescence of cells expressing SMO proteins with Anti SMO(A68V) antibody. HEK293T cells were transfected with pCDNA3-GFP SMO(WT) plasmid (left column) or pCDNA3-GFP SMO(A68V) plasmid (right column), then fixed and stained with Anti SMO(A68V) monoclonal antibody (Cat. #26425).


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