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Catalog Number: CST26425
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Gene Symbol: SMO; MRD21
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Description: Anti SMO(A68V) Mouse Monoclonal Antibody
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Background: Smoothened is a protein that in humans is encoded by the SMO gene. Smoothened is a G protein-coupled receptor that is a component of the hedgehog signaling pathway and is conserved from flies to humans. It is the molecular target of the teratogen cyclopamine. SMO can function as an oncogene. Activating SMO mutations can lead to unregulated activation of the hedgehog pathway and cancer
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Immunogen: A synthetic peptide from the internal region of SMO which includes the mutation of A68V, human origin.
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Applications: ELISA, WB, IHC
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Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
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Concentration: 1 mg/ml
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Host: Mouse
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Format: Liquid
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Clonality: Monoclonal
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Isotype:
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Purity: Purified from ascites
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Storage buffer:
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Preservative: no
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Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
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Species Reactivity: Recognizes A68V mutant, but not wild-type SMO of vertebrates.
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Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
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Western blot:
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