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Anti Vimentin Mouse Monoclonal Antibody
  • 品牌:Chemstan
  • 产地:China
  • 型号:100ug
  • 货号:CST26054
  • 价格: ¥2800/支
  • 发布日期: 2023-06-08
  • 更新日期: 2024-07-18
产品详请
产地 China
品牌 Chemstan
货号 CST26054
用途 Research Grade
纯度 >95% by SDS-PAGE.%
规格 100ug
是否进口
Gene Symbol:  VIM
Description:   Anti Vimentin Mouse Monoclonal Antibody
Background:   Vimentin is an intermediate filament protein found in various non-epithelial cells, especially in mesenchymal cells. It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.
Immunogen:   A synthetic peptide from the internal region of human vimentin protein.
Applications:   ELISA, WB, IHC, IP
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:100-1:1000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti vimentin antibody recognizes vimentin of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant vimentin protein. Purified His-tagged vimentin protein (Cat.#10190) was blotted with anti vimentin monoclonal antibody (Cat.# 26054).


image.png

Immunohistochemical analysis of  paraffin-embedded human renal cancer tissue  

with anti-vimentin monoclonal antibody (Cat.  #26054). Tissue samples were fixed 

with  formaldehyde and blocked with 1% serum for 15  min at 37 °C. Antigen retrieval was by heat  mediation in citrate buffer (pH6). Samples were  then incubated with primary antibody (1:100  dilution) overnight at 4°C.A HRP-conjugated  Goat anti-mouse IgG (1:50 dilution) was used as  secondary antibody

Note:
For research use only .

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