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| 产地 | China |
| 品牌 | Chemstan |
| 货号 | CST26054 |
| 用途 | Research Grade |
| 包装规格 | 100ug |
| 纯度 | >95% by SDS-PAGE.% |
| CAS编号 | |
| 是否进口 | 否 |
| Gene Symbol: VIM |
| Description: Anti Vimentin Mouse Monoclonal Antibody |
| Background: Vimentin is an intermediate filament protein found in various non-epithelial cells, especially in mesenchymal cells. It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression. |
| Immunogen: A synthetic peptide from the internal region of human vimentin protein. |
| Applications: ELISA, WB, IHC, IP |
|
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti vimentin antibody recognizes vimentin of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
|
Western blot analysis of recombinant vimentin protein. Purified His-tagged vimentin protein (Cat.#10190) was blotted with anti vimentin monoclonal antibody (Cat.# 26054). |
Immunohistochemical analysis of paraffin-embedded human renal cancer tissue
with anti-vimentin monoclonal antibody (Cat. #26054). Tissue samples were fixed
with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:100 dilution) overnight at 4°C.A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody
Note:
For research use only .
