Kit Components 
1. Anti active Gα13, Mouse Monoclonal Antibody (Catalog No. 26902): One vial – 22 μL (1mg/ml) in PBS,     pH 7.4, containing 50% glycerol and 0.05% sodium azide. This antibodyspecifically recognizes Gα13-GTP from all vertebrates.
2. Protein A/G Agarose (Catalog No. 30301): One vial – 400 μL of 50% slurry. 3. 5X Assay/Lysis Buffer  (Catalog No. 30303): One bottle – 30 mL of 250 mM Tris-HCl, pH 8, 750mM NaCl, 5 mM EDTA, 5%     Triton X-100.
4. Anti Gα13, Rabbit Polyclonal Antibody (Catalog No. 21005): One vial – 22 μL (1 mg/ml) inPBS, pH 7.4, contained 50% glycerol. 
Storage
Store all kit components at 4oC until their expiration dates.  
Materials Needed but Not Supplied
1. Stimulated and non-stimulated cell lysates
2. Protease inhibitors
3. 4 °C tube rocker or shaker
4. 1 M EDTA, pH8.0
5. 1 M MgCl2
6. 2X reducing SDS-PAGE sample buffer
7. Electrophoresis and immunoblotting systems
8. Immunoblotting wash buffer such as TBST (10 mM Tris-HCl, pH 7.4, 0.15 M NaCl, 0.05%Tween-20)
9. Immunoblotting blocking buffer (TBST containing 5% Non-fat Dry Milk or 3% BSA)
10. PVDF or nitrocellulose membrane
11. Secondary Antibody
12. ECL Detection Reagents 
Reagent Preparation
? 1X Assay/Lysis Buffer: Mix the 5X Stock briefly and dilute to 1X in deionized water. Just prior to
usage, add protease inhibitors such as 1 mM PMSF, 10 μg/mL leupeptin, and 10 μg/mL aprotinin. 
Note:
For research use only .