产地 | China |
品牌 | Chemstan |
货号 | APA133Mu01 |
用途范围 | Research Grade |
纯度 | % |
规格 | 10μg |
是否进口 | 否 |
产品名称
肿瘤坏死因子α(TNFa)活性蛋白
Active Tumor Necrosis Factor Alpha (TNFa)编号:APA133Mu01
物种:Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
缓冲液成份:20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
性状:冻干粉
纯度:> 95%
等电点:5.0
应用:Cell culture; Activity Assays.
Figure 1. Cell apoptosis of A549 cells after stimulated by TNFa. <br/>TNFa, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, inflammation and inhibit tumorigenesis. As reported, TNFa could inhibit the proliferation and induce apoptosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. A549 cells were incubated in DMEM with TNFa (1ng/mL, 10ng/mL) for 2h, 4h, 8h, 24h, 48h, then cells were observed by inverted microscope and IL-1β in cell supernatant was detected by ELISA. Cell apoptosis of A549 after incubation of 48h was shown in Figure 1.<br/> (A)A549 cells cultured in DMEM, stimulated with 1ng/mL TNFa for 48h; <br/>(B)A549 cells cultured in DMEM, stimulated with 10ng/mL TNFa for 48h; <br/>(C)A549 cells cultured in DMEM for 48h.
Table 1. ELISA detection of IL-1β expression from A549 cells by TNFa.
Effect of TNFa on the expression of IL-1β is shown in Table 1.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
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