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Active Nerve Growth Factor (NGF)
  • 品牌:Chemstan
  • 产地:China
  • 型号:10μg
  • 货号:APA105Hu01
  • 价格: ¥1440/支
  • 发布日期: 2023-12-12
  • 更新日期: 2024-02-23
产品详请
产地 China
品牌 Chemstan
货号 APA105Hu01
用途范围 Research Grade
纯度 %
规格 10μg
是否进口
产品名称
神经生长因子(NGF)活性蛋白
Active Nerve Growth Factor (NGF)
NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor
细胞因子神经科学

编号APA105Hu01

物种Homo sapiens (Human,人) 相同的名称,不同的物种。

缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)

性状冻干粉

纯度> 97%

等电点10.1

应用Cell culture; Activity Assays.

活性实验

Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. As reported, when the pheochromocytoma cell line PC12 is exposed to nerve growth factor (NGF), the cells respond over a period of a week by ceasing cell division and extending neurites (Greene and Tischler, 1976). The cells were grown in Ham's F12K containing 5% fetal calf serum and 10% horse serum on  polylysine or collagen coated plates. When cells reached log phase growth , fresh medium was added together with 10ng/mL of NGF, then cells were observed by inverted microscope everyday.
Cell division ceasing and differentiation of PC12 cells after incubation with NGF (10ng/mL) for 6 days was shown in Figure1.Control group which received no NGF displayed no neurite outgrowth and cells multiply rapidly.

NGF is also involved in pathways besides those regulating the life cycle of neurons. Besides, Alpha-2-Macroglobulin (a2M) has been identified as an interactor of NGF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human NGF and recombinant human a2M. Briefly, NGF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to a2M-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NGF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of NGF and a2M was shown in Figure 2, and this effect was in a dose dependent manner.
Figure. The binding activity of NGF with a2M

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

Note
For research use only .

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