产地 | China |
品牌 | Chemstan |
货号 | APA134Hu01 |
用途范围 | Research Grade |
纯度 | % |
规格 | 10μg |
是否进口 | 否 |
产品名称
肿瘤坏死因子β(TNFb)活性蛋白
Active Tumor Necrosis Factor Beta (TNFb)编号:APA134Hu01物种Homo sapiens (Human,人) 相同的名称,不同的物种。
缓冲液成份:20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
性状:冻干粉
纯度:> 97%
等电点:9.3
应用:Cell culture; Activity Assays.
TNF-β, a member of the tumor necrosis factor family, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells. The biological effects of TNF-β are very similar to TNF-α, due to the similarity of molecular structure and the receptors. As reported, TNF-β could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNF-β (10ng/mL) for 8h, 24h, 48h, 72h, then cells were observed by inverted microscope and IL-1β was detected in the cell supernatant by ELISA .
Cell necrosis after incubation with TNF-β(10ng/mL) for 72h was shown in Figure 1.
The production of IL-1β after incubation with TNF-β (10ng/mL) for 8h is shown in Table 1.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
Note
For research use only .
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