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Active Interleukin 1 Beta (lL1b)
  • 品牌:Chemstan
  • 产地:China
  • 型号:10μg
  • 货号:APA563Hu61
  • 价格: ¥2078/支
  • 发布日期: 2023-12-12
  • 更新日期: 2024-03-01
产品详请
产地 China
品牌 Chemstan
货号 APA563Hu61
用途范围 Research Grade
纯度 %
规格 10μg
是否进口

产品名称

白介素1β(IL1b)活性蛋白

Active Interleukin 1 Beta (IL1b)
IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
细胞因子肿瘤免疫感染免疫

编号:APA563Hu61

物种:Homo sapiens (Human,人) 相同的名称,不同的物种。

缓冲液成份:20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)

性状:冻干粉

纯度:> 95%

等电点:5.9

应用;Cell culture; Activity Assays.


活性实验

Interleukin-1 beta belongs to the interleukin 1 cytokine family, which plays a critical role in inflammation, immunity, antiviral responses, and a variety of diseases. It has been reported that IL-1β-induced IL-6 production is mediated by both PI3K and IRAK4 in A549 cells. To test the bioactivity of IL-1β, A549 cells were seeded into 24-well plate at a density of 1x105 cells/mL, and allowed to attach overnight before treated with or without certain concentrations (1ng/mL, 10ng/mL) of IL1-β for 4h and IL-6 levels in the cell supernatant were determined by ELISA.
IL-6 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL1-β, the data was shown in Table 1 and Figure 1.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。


Note
For research use only .

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