产地 | China |
品牌 | Chemstan |
货号 | APA563Hu61 |
用途范围 | Research Grade |
纯度 | % |
规格 | 10μg |
是否进口 | 否 |
产品名称
白介素1β(IL1b)活性蛋白
Active Interleukin 1 Beta (IL1b)编号:APA563Hu61
物种:Homo sapiens (Human,人) 相同的名称,不同的物种。
缓冲液成份:20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
性状:冻干粉
纯度:> 95%
等电点:5.9
应用;Cell culture; Activity Assays.
Interleukin-1 beta belongs to the interleukin 1 cytokine family, which plays a critical role in inflammation, immunity, antiviral responses, and a variety of diseases. It has been reported that IL-1β-induced IL-6 production is mediated by both PI3K and IRAK4 in A549 cells. To test the bioactivity of IL-1β, A549 cells were seeded into 24-well plate at a density of 1x105 cells/mL, and allowed to attach overnight before treated with or without certain concentrations (1ng/mL, 10ng/mL) of IL1-β for 4h and IL-6 levels in the cell supernatant were determined by ELISA.
IL-6 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL1-β, the data was shown in Table 1 and Figure 1.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
Note
For research use only .
15926423062
武汉科斯坦生物科技有限公司 版权所有(C)2024 XML 技术支持: 盖德化工网 食品商务网
武汉科斯坦生物科技有限公司