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Active Osteopontin(OPN)
  • 品牌:Chemstan
  • 产地:China
  • 型号:10μg
  • 货号:APA899Hu61
  • 价格: ¥556/支
  • 发布日期: 2023-12-12
  • 更新日期: 2024-02-23
产品详请
产地 China
品牌 Chemstan
货号 APA899Hu61
用途范围 Research Grade
纯度 %
规格 10μg
是否进口

产品名称

骨桥素(OPN)活性蛋白

Active Osteopontin (OPN)
SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin
肿瘤免疫骨骼代谢

编号:APA899Hu61

物种:Homo sapiens (Human,人) 相同的名称,不同的物种。

缓冲液成份:20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)

性状:冻干粉

纯度:> 95%

等电点4.3

应用:Cell culture; Activity Assays.

活性实验

Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which would promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappa B pathways in MCF-7 breast cancer cell line. Briefly, MCF-7 cells were seeded overnight at a density of 1x105 cells/mL, and treated with or without OPN(100ng/ml、200ng/ml) for 24h and MCP-1 levels in the cell supernatant were determined by ELISA.
MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN, the data was shown in Table 1 and Figure 1.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

Note
For research use only .

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