产地 | China |
品牌 | Chemstan |
货号 | APA899Hu61 |
用途范围 | Research Grade |
纯度 | % |
规格 | 10μg |
是否进口 | 否 |
产品名称
骨桥素(OPN)活性蛋白
Active Osteopontin (OPN)编号:APA899Hu61
物种:Homo sapiens (Human,人) 相同的名称,不同的物种。
缓冲液成份:20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
性状:冻干粉
纯度:> 95%
等电点:4.3
应用:Cell culture; Activity Assays.
Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which would promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappa B pathways in MCF-7 breast cancer cell line. Briefly, MCF-7 cells were seeded overnight at a density of 1x105 cells/mL, and treated with or without OPN(100ng/ml、200ng/ml) for 24h and MCP-1 levels in the cell supernatant were determined by ELISA.
MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN, the data was shown in Table 1 and Figure 1.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
Note
For research use only .
15926423062
武汉科斯坦生物科技有限公司 版权所有(C)2024 XML 技术支持: 盖德化工网 食品商务网
武汉科斯坦生物科技有限公司