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Active Lemur Tyrosine Kinase 3(LMTK3)
  • 品牌:Chemstan
  • 产地:China
  • 型号:10μg
  • 货号:APE375Hu01
  • 发布日期: 2023-12-12
  • 更新日期: 2024-06-17
产品详请
产地 China
品牌 Chemstan
货号 APE375Hu01
用途 Research Grade
纯度 > 95%
规格 10μg
是否进口

产品名称

Lemur酪氨酸激酶3(LMTK3)活性蛋白

Active Lemur Tyrosine Kinase 3 (LMTK3)
LMR3; TYKLM3; Serine/threonine-protein kinase LMTK3

编号:APE375Hu01
物种:Homo sapiens (Human,人) 相同的名称,不同的物种。
缓冲液成份:20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
性状:冻干粉
纯度:> 95%
等电点:6.3
应用:Cell culture; Activity Assays.

活性实验

Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance. It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line. To test the bioactivity of LMTK3, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of LMTK3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C.
Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.

The dose-effect curve of LMTK3 was shown in Figure 2. It was obvious that LMTK3 significantly promoted cell proliferation of MCF-7 cells. The ED50 for this effect is approximately 6.14~50ng/mL.


用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

Note
For research use only .


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