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Active Interleukin 2 (IL2)
  • 品牌:Chemstan
  • 产地:China
  • 型号:10ug
  • 货号:APA073Ra61
  • 价格: ¥1814/支
  • 发布日期: 2023-12-12
  • 更新日期: 2024-11-12
产品详请
产地 China
品牌 Chemstan
货号 APA073Ra61
用途 Research Grade
包装规格 10ug
纯度 %
CAS编号
是否进口
产品名称
白介素2(IL2)活性蛋白
Active Interleukin 2 (IL2)
TCGF; Lymphokine; T-Cell Growth Factor; Aldesleukin
编号:APA073Ra61
物种:Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
缓冲液成份:PBS, pH7.4, containing 5% Trehalose.
性状:冻干粉  纯度:> 90%   等电点:6.3
应用:Cell culture; Activity Assays.


IL-2(Interleukin-2) is a cytokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2 is a type of signaling molecule in the immune system, that is required for both T-cell and B-cell proliferation and other activities crucial to regulation of the immune response.Therefore, in order to detect the bioactivity of recombinant rat IL-2, spleen single suspensions were prepared, activated with conA (final concentration 3 ug/ml). Cells were collected after 72h and washed with hanks.Then mouse splenic lymphocytes were were seeded into triplicate wells of 96-well plates at a density of 10,000 cells/well with or without the addition of various concentrations of recombinant rat IL-2. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). 10 μl of CCK-8 solution was added to each well of the plate, the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C . Proliferation of Splenic lymphocytes cells after incubation with IL-2 for 72h observed by inverted microscope was shown in Figure 1.

Figure 2. The dose-effect curve of IL-2 on Splenic lymphocytes cells.


用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
Note
For research use only .

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