Introduction:
The Chemi - Trans™ RNAiBor siRNA Transfection Reagent is a novel, high
biological compacity and biodegradable nanoparticle-based in vitro small
RNA (e. g., siRNA, miRNA mimics and miRNA inhibitor) delivery tool, which
provides a robust transfection efficiency in a variety of commonly used and
hard-to-transfect mammalian cells, such as adherent and suspension cells,
as well as primary cells, but with less cytotoxicity in comparison with other
lipid-base transfection reagent as known in the market.
Advantages:
? Robust transfection efficiency and low cytotoxicity.
? Excellent for some primary cell lines, such primary fibroblast cells,
primary neuron cells, macrophage cells, T cells and stem cells.
? Compatible with serum and antibiotics in growth medium.
Important Guidelines for Transfection:
(1) For a robust transfection efficiency, the siRNA (PAGE or HPLC purifiedgrade) is recommended. Never fail to use siRNA preserved in water or
desalted and lyophilized condition.
(2) It is strictly prohibited to dilute both the Chemi - Trans™ RNAiBor siRNATransfection Reagent and the siRNA solution by Serum-free medium ordilution buffer in preparation of the transfection complexes, just to mixthe Chemi - Trans™ Reagent and siRNA solution directly as the ratio of
1:1.
For Research Use Only. Not for use in diagnostic procedures.
