Introduction:
The Chemi - Trans™ Lipofectin II Transfection Reagent is a lipid-based
transfection reagent that forms a complex with DNA or small RNA, and can
efficiently deliver the complex into a variety of adherent and suspension
mammalian cell lines. Chemi - Trans™ Lipofectin II Transfection Reagent
has been tested to get a good performance as well as Lipofectamine® 3000
Reagent in DNA and siRNA transfection,
Advantages:
? Robust transfection efficiency and low cytotoxicity.
? Excellent for difficult-to-transfection cells, such as some primary cells,
macrophage cells, suspension cell lines and stem cells.
? Compatible with serum and antibiotics in culture medium.
Important Guidelines for Transfection:
(1) For maximum transfection efficiency, using serum-free medium (such as
Opti-MEM® Reduced Serum Medium) to dilute DNA and the Chemi -
Trans™ Lipofectin II Transfection Reagent is a must.
(2) While the standard protocols for DNA transfection being given below,
optimization is often needed for maximal transfection efficiency.
(3) It is unnecessary to wash cells and change medium after transfection.
For Research Use Only. Not for use in diagnostic procedures.
