Introduction:
The Chemi - Trans™ RNAiMAX Transfection Reagent is a high efficiency
RNA transfection reagent, derived from a type of natural compound and
specifically designed for small RNA oligonucleotides (e. g, siRNA and
miRNA.). This reagent can provide the robust transfection efficiency in a
variety of commonly used and hard-to-transfect mammalian cells. Such as
adherent and suspension cells, as well as primary cells.
Advantages:
? Robust transfection efficiency and low cytotoxicity.
? Excellent for small RNA transfection.
? Compatible with serum and antibiotics in culture medium.
Important Guidelines for Transfection:
(1) For maximum transfection efficiency, using serum-free medium (such as
Opti-MEM® Reduced Serum Medium) to dilute siRNA/miRNA mimics or
miRNA inhibitor and the Chemi -Trans™ RNAiMAX Transfection Reagent is a
must.
(2) While the standard protocols for siRNA transfection being given below,
optimization is often needed for maximal transfection efficiency.
(3) It is unnecessary to wash cells and change medium after transfection.
For Research Use Only. Not for use in diagnostic procedures.
