1.Alternative Name:LEU3;CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
2.Target:CD4
3.Source:Human CD4 was expressed from HEK293 with poly His at the C-terminal
4.Sequence:Lys26-Gln390
5.Protein structure:P01730His
6.Molecular Characterization:The protein has a calculated MW of 42kDa.The reducing (R) heterodimer protein migrates as 40-60 kDa due to glycosylation
7.Purity:SDS-PAGE>95%，SEC>95%
8.Reconstitution:sterile H2O
9.Endotoxin:<1EU/mg
10.Formulation:Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
Note:For research use only .


11.SDS-PAGE

Determined by SDS-PAGE, the purity of the protein is greater than 95%.

12.SEC-HPLC

Determined by SEC-HPLC, the purity of the protein is greater than 95%.

13.ELISA

When Recombinant Human CD4/His Protein is immobilized at 2μg/mL (100 μL/well), the concentration of Recombinant Anti-Human CD4 (Ibalizumab) that produces 50% of the optimal binding response is approximately 70 ng/ml.

14.SPR

Recombinant Anti-Human CD4 (Ibalizumab)（Cat.No.B554401）captured on Protein A chip , can bind Recombinant Human CD4/His Protein（Cat.No.B21656903）with a KD constant of 2.42E-11 M, determined in a SPR assay .