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| 产地 | 中国 |
| 保存条件 | |
| 品牌 | Chemstan |
| 货号 | CS20860 |
| 用途 | 科研用途 |
| 检测方法 | |
| 保质期 | |
| 适应物种 | |
| 检测限 | |
| 数量 | |
| 包装规格 | 96T |
| 标记物 | |
| 样本 | |
| 应用 | |
| 是否进口 | 否 |
关键信息(Key Features):
|
Full Name |
Free Triiodothyronine |
|
Synonym |
fT3 |
|
Assay Type |
Competitive |
|
Reactivity |
Universal |
|
Range |
1.56-100pg/mL |
|
Sensitivity |
0.93pg/mL |
|
Sample Type |
Serum,plasma and other biological fluids |
|
Sample volume |
50μL |
|
UNIProt ID |
|
|
Microplate |
96-wells plate breakable into 12 x 8 wells strip |
反应原理(Test Principle)：
本试剂盒采用竞争ELISA法。用fT3抗原包被于酶标板上，实验时样品或标准品中的fT3与包被的fT3竞争生物素标记的抗fT3单抗上的结合位点，游离的成分被洗去。加入HRP酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在HRP酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，fT3浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中fT3的浓度。
存储(Storage)：
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
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典型数据(Typical Data): |
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fT3 ELISA Standard Curve Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting. |
