产地 | 中国 |
保存条件 | |
品牌 | Chemstan |
货号 | CS20856 |
用途 | 科研用途 |
检测方法 | |
保质期 | |
适应物种 | |
检测限 | |
数量 | |
包装规格 | 96T |
标记物 | |
样本 | |
应用 | |
是否进口 | 否 |
关键信息(Key Features):
Full Name |
Cyclic adenosine monophosphate |
Synonym |
Cyclic adenosine monophosphate;cAMP;cyclic AMP;3'-5'-cyclic adenosine monophosphate; |
Assay Type |
Competitive |
Reactivity |
Universal |
Range |
1.56-100ng/mL |
Sensitivity |
0.93ng/mL |
Sample Type |
Serum,plasma and other biological fluids |
Sample volume |
50μL |
UNIProt ID |
|
Microplate |
96-wells plate breakable into 12 x 8 wells strip |
反应原理(Test Principle):
本试剂盒采用竞争ELISA法。用cAMP抗原包被于酶标板上,实验时样品或标准品中的cAMP与包被的cAMP竞争生物素标记的抗cAMP单抗上的结合位点,游离的成分被洗去。加入HRP酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在HRP酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,cAMP浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中cAMP的浓度。
存储(Storage):
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
典型数据(Typical Data): |
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cAMP ELISA Standard Curve Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting. |